Protocol for analyzing protein liquid–liquid phase separation

Aqueous two-phase systems for protein separation: phase separation and applications.

Aqueous two-phase systems (ATPS) that are formed by mixing a polymer (usually polyethylene glycol, PEG) and a salt (e.g. phosphate, sulphate or citrate) or two polymers, and water can be effectively used for the separation and purification of proteins. The partitioning between both phases is dependent on the surface properties of the proteins and on the composition of the two phase system as ha...

Analyzing Protocol Implementations for Interoperability

We propose PIC, a tool that helps developers search for non-interoperabilities in protocol implementations. We formulate this problem using intersection of the sets of messages that one protocol participant can send but another will reject as non-compliant. PIC leverages symbolic execution to characterize these sets and uses two novel techniques to scale to real-world implementations. First, it...

Phase Separation in Multicomponent Aqueous-Protein Solutions

published in Advance ACS Abstracts, December 1, 1994. 0022-3654/95/2099-0454$09.00/0 we present here the results of experimental and theoretical studies of a ternary solution constituted of the proteins yma (designated as protein A), a “high-Tc” protein, and yw (designated as protein B), a “low-Tc” protein, in aqueous solution. The solution pH was kept constant at 7.1 by a 100 mM phosphate buff...

Practical Techniques for Improving the Performance of Polymeric Membranes and Processes for Protein Separation and Purification

Protein separation and purification technologies play an essential role in various industries including but not limited to pharmaceuticals, dairy as well as the food sector. Accordingly, a wide variety of techniques such as chromatography and electrophoresis has been developed and utilized extensively over the years for this purpose. Despite their widespread acceptance, conventiona...

Strategies for Protein Separation